Movie 3

Growth of scd1^low and scd1^low tea1Δ cells expressing wild-type Cdc2. CRIB-3mCitrine distribution and cell morphology in exponentially-growing scd1^low and scd1^low tea1Δ cells expressing wild-type Cdc2. Thiamine was added to repress scd1 expression just after the first time-point, which is paused in the movie. Cells correspond to those shown in Fig. S2. Effects of scd1 repression, including isotropic-like growth of scd1^low tea1Δ cells, become apparent about half-way through the movie. See Fig. S2 legends and Methods for further details. Time interval during acquisition, 9 min; total elapsed time, 540 min; time compression at 15 frames per second playback, 8100X.