Movie 7

Inhibition of Pom1 kinase activity in scd1Δ cells leads to PORTLI growth. Cell morphology and distribution of Pom1-tdTomato and GFP-Bgs4, or Pom1-as1-tdTomato and GFP-Bgs4, in scd1Δ cdc2-asM17 genetic background after 3-BrB-PP1 treatment. Bgs4 on the plasma membrane indicates sites of growth. Cells correspond to those shown in Fig. 6A. 3-BrB-PP1 inhibits activity of both Cdc2-asM17 and Pom1-as1-tdTomato and was added just after the first time-point. Note that 3-BrB-PP1 treatment depolarizes Pom1-as1-tdTomato, and this leads to PORTLI growth. Time interval during acquisition, 20 min; total elapsed time, 240 min; time compression at 15 frames per second playback, 18,000X.