Movie 9

Deletion of rga4 leads to PORTLI growth in scd1Δ cells. mCherry-Bgs4 distribution and cell morphology of scd1Δ and scd1Δ rga4Δ mutants in cdc2-asM17 mCherry-bgs4 background. Bgs4 on the plasma membrane indicates sites of growth. Cells correspond to those shown in Fig. 7A, with slightly larger fields. Cdc2 kinase activity was inhibited by 3-BrB-PP1 addition 30 min before imaging. Time interval during acquisition, 12 min; total elapsed time, 480 min; time compression at 15 frames per second playback, 10,800X.