Supplemental movie 4

Time-lapse movie of living cwh43-G753R mutant cells. Cells were first cultivated at 26°C, and then shifted to 36°C in a microfluidic perfusion chamber with a continuous supply of EMM2 medium. Differential interference contrast (DIC) images were obtained at 15-min intervals and are shown at 8 frames / sec. The same cells are shown in Figure 2B (bottom).

The putative ceramide-conjugation protein Cwh43 regulates G0 quiescence, nutrient metabolism and lipid homeostasis in fission yeast

Norihiko Nakazawa, Takayuki Teruya, Kenichi Sajiki, Kazuki Kumada, Alejandro Villar-Briones, Orie Arakawa, Junko Takada, Shigeaki Saitoh, and Mitsuhiro Yanagida

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.217331