Movie S5

Image sequence depicts PMNs pre-stained with CellMask Deep Red (purple) to label Plasma membrane and CellTracker CMPTX Red (red) to label cytoplasm for 15 mins before stimulating with fMLF-Alexa Fluor 488 (green; 50 nM). A z-stack (~6 μm from the bottom) was obtained after 5 mins of stimulation from live cells, surface rendered and different channels were sequentially added or removed using animation tool in Imaris.

The LTB4–BLT1 axis regulates the polarized trafficking of chemoattractant GPCRs during neutrophil chemotaxis

Bhagawat C. Subramanian, Konstadinos Moissoglu, and Carole A. Parent

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.217422