Movie 5

FRAP experiment on CHMP2B reticulum.
The movie shows FRAP experiments on GUVs incubated with either CHMP2B-ΔC (left) or CHMP2B-FL (right), in condition of GUV partial coverage. A region corresponding to the upper spherical cap of the GUV is shown.

The ESCRT protein CHMP2B acts as a diffusion barrier on reconstituted membrane necks

Nicola De Franceschi, Maryam Alqabandi, Nolwenn Miguet, Christophe Caillat, Stephanie Mangenot, Winfried Weissenhorn, and Patricia Bassereau

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.217968