Movie S3

MT1-MMP is exocytosed from flotillin-positive vesicles
MDA-MB-231 cells that co-express flotillin 2-mCherry and MT1-MMP-pHluorin were monitored by using confocal microscopy; 1 frame/3 seconds. A flotillin 2-containing vesicle is indicated by a red arrow that becomes yellow when the GFP fluorescent signal appears, indicating that MT1-MMP is exocytosed. Red open arrows indicate flotillin 2-positive vesicles converging towards the exocytic site. Scale bar: 2 μm.

MT1-MMP targeting to endolysosomes is mediated by upregulation of flotillins

Damien Planchon, Eduardo Rios Morris, Mallory Genest, Franck Comunale, Sophie Vacher, Ivan Bièche, Evgeny V. Denisov, Lubov A. Tashireva, Vladimir M. Perelmuter, Stefan Linder, Philippe Chavrier, Stéphane Bodin, and Cécile Gauthier-Rouvière

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.218925