Movie S3

Representative super-resolution movie of a sperm, which at the beginning of the recording possesses the septum F-actin structure. The F-actin structure in the septum region depolymerizes prior to the initiation of the AR after addition of 10 μM ionomycin. Related to Figure 5A.

Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis

Ana Romarowski, Ángel G. Velasco Félix, Paulina Torres Rodríguez, María G. Gervasi, Xinran Xu, Guillermina M. Luque, Gastón Contreras-Jiménez, Claudia Sánchez-Cárdenas, Héctor V. Ramírez-Gómez, Diego Krapf, Pablo E. Visconti, Dario Krapf , Adán Guerrero, Alberto Darszon, and Mariano G. Buffone

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.218958