Movie S4

Representative super-resolution movie of a sperm, which at the beginning of the recording possesses the lower acrosome F-actin structure. The F-actin structure in the lower acrosome region de-polymerizes prior to the initiation of the AR after the addition of 10 μM ionomycin. Related to Figure 5D.

Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis

Ana Romarowski, Ángel G. Velasco Félix, Paulina Torres Rodríguez, María G. Gervasi, Xinran Xu, Guillermina M. Luque, Gastón Contreras-Jiménez, Claudia Sánchez-Cárdenas, Héctor V. Ramírez-Gómez, Diego Krapf, Pablo E. Visconti, Dario Krapf , Adán Guerrero, Alberto Darszon, and Mariano G. Buffone

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.218958