Movie S5

Live-cell imaging of cortical actin cytoskeleton and plasma membrane reorganization during AR. Representative super-resolution movie of a sperm loaded with SiR-actin and FM4-64 showing the formation of a hybrid vesicle at the onset of the AR. Each super-resolution image was computed by analyzing 300 wide-field images through SRRF analysis. Related to Figure 6D-E.

Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis

Ana Romarowski, Ángel G. Velasco Félix, Paulina Torres Rodríguez, María G. Gervasi, Xinran Xu, Guillermina M. Luque, Gastón Contreras-Jiménez, Claudia Sánchez-Cárdenas, Héctor V. Ramírez-Gómez, Diego Krapf, Pablo E. Visconti, Dario Krapf , Adán Guerrero, Alberto Darszon, and Mariano G. Buffone

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.218958