Movie S4

Association of Bim1 with MTs in lysate. A zoomed-in view of Bim1-TagRFP-T (magenta) associated with MTs (green) along their length and accumulated at the plus ends from lysate of cells arrested in S phase. Rhodamine-labeled seeds are also in magenta at the minus ends. Corresponds to kymographs in Figure 3A. Scale bar is 2 μm. Playback is 20fps.

Microtubule dynamics regulation reconstituted in budding yeast lysates

Zane J. Bergman, Jonathan Wong, David G. Drubin, and Georjana Barnes

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.219386