Translocation of Kip3 along MTs in Lysate. Kip3-TagRFP-T (traveling dots) and rhodamine-labeled seeds (bright bars) on the left and GFP-tubulin MTs on the right from lysate of cells arrested in metaphase. Kip3-TagRFP-T puncta bound along the MT and moved towards the plus end. Corresponds to kymographs in Figure S1. Scale bar is 2 μm. Playback is 20fps.