To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie S3 (Related to Fig. 4B). 3D-SIM reconstruction of PLK4 MTOCs in Xenopus egg extracts using rhodamine-tubulin. Reconstruction of the whole aster. PLK4 is a microtubule-associated protein that self-assembles promoting de novo MTOC formation Susana Montenegro Gouveia, Sihem Zitouni, Dong Kong, Paulo Duarte, Beatriz Ferreira Gomes, Ana Laura Sousa, Erin M. Tranfield, Anthony Hyman, Jadranka Loncarek, and Monica Bettencourt-Dias J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.219501 Download Original Video