Movie 3

Transport of vesicles colabeled with LGI1-GFP and ADAM23-mCherry in a proximal axon
A neuron co-transfected with LGI1-GFP and ADAM23-mCherry at DIV8. Vesicle transport visualized 16 h after transfection using two-channel overlay recording. Streamed time-lapse images were acquired with 40 frames per 60 s for 90 s. Movie is played at 5.3 frames per second. Note the bidirectional transport of double-labeled vesicles.