Movie 5

Transport of vesicles colabeled with TAG-1-GFP and ADAM23-mCherry in the proximal axonal region
A neuron co-transfected at DIV8 with TAG-1-GFP and ADAM23-mCherry. Axonal transport visualized 16 h after transfection using two-channel overlay recording. Streamed time-lapse images were acquired with 40 frames per 60 s for 132 s. Movie is played at 5.3 frames per second. Note that double-labeled vesicles moved mostly retrogradely.