Transport of vesicles colabeled with TAG-1-GFP and ADAM23-mCherry in the distal axonal region
A neuron co-transfected at DIV8 with TAG-1-GFP and ADAM23-mCherry. Axonal transport visualized 16 h after transfection using two-channel overlay recording. Streamed time-lapse images were acquired with 40 frames per 60 s for 116 s. Movie is played at 5.3 frames per second. Note the bidirectional transport of the double-labeled vesicles.