Movie 7

Axonal transport vesicles containing Caspr2-GFP and ADAM23-mcherry
A neuron co-transfected at DIV8 with Caspr2-GFP and ADAM23-mCherry. Axonal transport visualized 16h after transfection using two-channel overlay recording. Streamed time-lapse images were acquired with 40 frames per 60 s for 210 s. Movie is played at 5.3 frames per second. In this axon, five double-labeled vesicles are observed moving in the retrograde direction.