Movie 1

Actin network assembly on a VCA-functionalized SLB.
Growth of actin (1 μM) on an SLB functionalized with VCA in the presence of 10 nM Arp2/3 and 100 μM ATP is visualized using TIRF microscopy. The movie spans a time period of 20 minutes and shows VCA intensity (left) and actin intensity (right) on the surface. Time is measured from the mixing of reaction components. Intensities are color-coded.