Movie 4

ATP is required for myosin-driven breakdown of actin networks.
Myosin-driven breakdown of actin network stalls at bundling in the absence of the ATP regeneration system. 100 μM ATP was provided as in a standard reaction mix. The first flash indicates the addition of myosin and the second flash indicates the addition of the ATP regeneration system. Video shows actin intensity in grayscale and time is measured from the addition of myosin.