Movie 8

Buckling and rupture of actin bundles in the dynamic steady state distribution
Actin dynamics observed in the steady state actin distribution in a pulse-chase experiment (Fig. 5B). Actin bundles with homogeneous distribution of Label1 (magenta) are speckled with Label2 (green) monomers. The movie shows a 40 second time window, captured at 104 minutes after the addition of myosin. Images have been background subtracted by using a walking average over 3 frames.

Myosin-II activity generates a dynamic steady state with continuous actin turnover in a minimal actin cortex

Sonal, Kristina A. Ganzinger, Sven K. Vogel, Jonas Mücksch, Philipp Blumhardt, and Petra Schwille

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.219899