Movie 10

Evolution of actin and keratin cytoskeleton self-organization in keratinocytes in the chiral actin pattern
Representative time-lapse series showing cytoskeleton progression in latrunculin A-treated HaCaT cells expressing mEmerald-Keratin14 with mRuby-Lifeact seeded on 1800 μm2 fibronectin-coated islands. 20 nM of latrunculin A was added to cells 8 h after seeding. Scale bar: 10 μm. Timestamp: hh:mm. Playback rate: 10 frames sec-1.

Actin cytoskeleton self-organization in single epithelial cells and fibroblasts under isotropic confinement

Salma Jalal, Shidong Shi, Vidhyalakshmi Acharya, Ruby Yun-Ju Huang, Virgile Viasnoff, Alexander D. Bershadsky, and Yee Han Tee

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.220780