Movie 3

Evolution of actin cytoskeleton self-organization in non-keratinocyte epithelial cells after epithelial-mesenchymal transition
Representative time-lapse series showing actin cytoskeleton development in NBT-II cells expressing Lifeact-GFP pretreated with 100 ng Ml^-1 Epidermal Growth Factor (EGF) for 3 days and replated on 1800 μm² fibronectin-coated islands in the continued presence of EGF. Scale bar: 10 μm. Timestamp: hh:mm. Playback rate: 20 frames sec^-1.