Movie 7

Representative time-lapse series showing actin cytoskeleton development and nucleus rotation in diploid (left, untreated cells) or tetraploid (right, achieved by pre-treatment with cytochalasin D) HFF cells expressing GFP-Lifeact with BFP-NLS seeded on an 1800 μm2 fibronectin-coated island. Scale bar: 10 μm. Timestamp: hh:mm. Playback rate: 10 frames sec-1.

Actin cytoskeleton self-organization in single epithelial cells and fibroblasts under isotropic confinement

Salma Jalal, Shidong Shi, Vidhyalakshmi Acharya, Ruby Yun-Ju Huang, Virgile Viasnoff, Alexander D. Bershadsky, and Yee Han Tee

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.220780