Rotation of the nucleus in latrunculin A-treated keratinocytes
Representative time-lapse series showing actin cytoskeleton development and nucleus rotation in latrunculin A-treated HaCaT cells expressing GFP-Lifeact with RFP-H1 seeded on a 2000 μm2 fibronectin-coated island. 200 nM of latrunculin A was added to cells 40 minutes after seeding. Scale bar: 10 μm. Timestamp: hh:mm. Playback rate: 10 frames sec-1.