Movie 4

Examples of filopodial shaft and base adhesions during cell spreading U2OS cell co-transfected with mApple-F-tractin (red) and GFP-paxillin (white), plated on fibronectin-treated coverslip for 18 min before imaging. Airyscan microscopy with a single ventral image acquired every 30 sec for 12 min. First part of movie shows both channels, second part shows GFP-paxillin (white) alone. Cyan arrow indicates shaft adhesion, green arrow indicates base adhesion. Corresponds with Fig. 7A. Scale bar 2 μm.