Movie 5

Example of simultaneous filopodial and adhesion assembly during cell spreading U2OS cell co-transfected with mApple-F-tractin (red) and GFP-paxillin (white), plated on fibronectin-treated coverslip for 20 min before imaging. Airyscan microscopy with a single ventral image acquired every 30 sec for 4 min. First part of movie shows both channels, second part shows GFP-paxillin (white) alone. Red arrow indicates location of simultaneous filopodia and adhesion assembly. Corresponds with Fig. 7B. Scale bar 1 μm.