Movie 6

Example of filopodium assembles without an accompanying new adhesion assembly during cell spreading U2OS cell co-transfected with mApple-F-tractin (red) and GFP-paxillin (white), plated on fibronectin-treated coverslip for 16 min before imaging. Airyscan microscopy with a single ventral image acquired every 30 sec for 2 min. First part of movie shows both channels, second part shows GFP-paxillin (white) alone. Orange arrow indicates location of filopodia assembly with no adhesion assembly. Corresponds with Fig. 7C. Scale bar 0.5 μm.