Movie 7

Single-cell imaging of YFP-PML-IV dynamics at the transgene array in HeLa, HI 1-1/ YFP-PML-IV cells. Time series of maximum projections of image stacks of YFP-PML-IV (green), Cherry-lac repressor (red) and differential interference contrast (DIC) images of HeLa HI 1-1/YFP-PML-IV cells collected every 30 minutes. Movie display rate is 1 frame per second.

PML is recruited to heterochromatin during S phase and represses DAXX-mediated histone H3.3 chromatin assembly

Prashanth Krishna Shastrula, Isabel Sierra, Zhong Deng, Frederick Keeney, James E. Hayden, Paul M. Lieberman, and Susan M. Janicki

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.220970