Movie 1

Time lapse imaging of a splitting event corresponding to Fig. 2A. C2C12 were transfected with Golgi-CFP (red) and Sec23a-EYFP (green), induced to differentiate and imaged at the transition from phase 1 to 2 every 10-sec in the multi-position time-lapse spinning disk confocal microscope. Z-stacks covering the entire cell were acquired and sum projections are reported. The arrowheads and the arrows point towards 2 distinct splitting Golgi structures.

GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation

Emiliana Giacomello, Paolo Ronchi, and Rainer Pepperkok

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.222083