Time lapse imaging of BFA treatment in differentiating C2C12 cells. C2C12 cells were transfected with Golgi-CFP (red) and Sec23a-EYFP (green), induced to differentiation and treated with BFA. Cells imaged in the multi-position time-lapse spinning disk confocal microscope every 5 minutes. Z-stacks covering the entire cell were acquired and sum projections are reported.