Movie 1

Localization of exocyst subunits Sec6, Sec3a, Sec5b and Sec5d during division of caulonemal apical cells expressing mCherry-α-tubulin and a GFP-tagged version of the indicated exocyst subunit.
Reference images that show the progression of phragmoplast development, visualized with GFP-α-tubulin, are depicted in the middle row. A time sequence showing cell plate development, visualized with cell plate marker SCAMP4 (de Keijzer et al. 2017) is depicted on the right as a reference. Time after anaphase onset (t=0) is indicated in min:sec. Images are maximum z-projections of 3 confocal planes spaced 0.5 μm apart. Scalebar, 5 μm.