Movie 3
Sec6 partially co-localizes with MAP65 on antiparallel microtubule overlaps in caulonemal cells expressing citrine labelled MAP65 and Sec6-mCherry.
In a Δkin4-Ic genetic background, microtubule overlaps are extended and the partial co-localization is more pronounced. Time with respect to anaphase onset (t=0) is indicated in min:sec. Images are maximum z-projections of 3 confocal planes spaced 0.5 μm apart. Scalebar, 5 μm.
Exocyst subunit Sec6 is positioned by microtubule overlaps in the moss phragmoplast prior to cell plate membrane arrival
J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.222430