Movie 3

Sec6 partially co-localizes with MAP65 on antiparallel microtubule overlaps in caulonemal cells expressing citrine labelled MAP65 and Sec6-mCherry.
In a Δkin4-Ic genetic background, microtubule overlaps are extended and the partial co-localization is more pronounced. Time with respect to anaphase onset (t=0) is indicated in min:sec. Images are maximum z-projections of 3 confocal planes spaced 0.5 μm apart. Scalebar, 5 μm.