Movie 4

Time sequence of dividing caulonemal cells expressing Sec6-GFP and mCherry-α-tubulin with silencing of MAP65a, b and c non-induced (control) and induced though the addition of β-estradiol.
During MAP65a, b and c silencing, the localization of exocyst subunit Sec6 to antiparallel microtubule overlaps becomes patchy. Time with respect to anaphase onset (t=0) is indicated in min:sec. Images are maximum z-projections of 3 confocal planes spaced 0.5 μm apart. Scalebar, 5 μm.