Movie 6

Dividing cells with membrane marker SCAMP4-mCherry in Sec6 RNAi without or with RNAi induction by β-estradiol treatment show that the initial recruitment of membrane materials is reduced upon Sec6 silencing.
The intensity of SCAMP4-mCherry is depicted with the same contrast settings. Time with respect to anaphase onset (t=0) is indicated in min: sec. Images are maximum z-projections of 3 planes spaced 0.5 μm apart acquired in the central plane. Scale bar, 5 μm.