MCF10A cells stably expressing GFP-DLC3-K725E were pretreated with TGF-β for 7 days, transfected with pmOrange2-C1-Endo RhoB and, on the next day, replated onto collagen-coated coverslips. 24 h later, cells were imaged by TIRF live microscopy. Time in seconds. Scale bar, 2 μm.
Bettina Noll, David Benz, Yannick Frey, Florian Meyer, Manuel Lauinger, Stephan A. Eisler, Simone Schmid, Peter L. Hordijk, and Monilola A. Olayioye
J Cell Sci
2019. 132:None-None; doi: 10.1242/jcs.223172