Movie 1

MCF10A cells stably expressing GFP-DLC3-K725E were pretreated with TGF-β for 7 days, transfected with pmOrange2-C1-Endo RhoB and, on the next day, replated onto collagen-coated coverslips. 24 h later, cells were imaged by TIRF live microscopy. Time in seconds. Scale bar, 2 μm.