Movie 3

3D reconstruction of Figure 1C showing TNT connecting RAW/LR5 macrophages with MDA-MB-231 tumor cells. Cells are stained with WGA (green) to label the membrane, and F-actin (magenta). Images were acquired using a Nikon Structured Illumination N-SIM system on an inverted Nikon ECLIPSE Ti-E equipped with a 100× 1.49 NA objective. 3D reconstruction was generated using Imaris software calibrated and maintained by the Analytical Imaging Facility at Albert Einstein College of Medicine.

Tunneling nanotubes, a novel mode of tumor cell–macrophage communication in tumor cell invasion

Samer J. Hanna, Kessler McCoy-Simandle, Edison Leung, Alessandro Genna, John Condeelis, and Dianne Cox

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.223321