Movie 5

Time-lapse imaging of GFP-CAAX RAW/LR5 macrophages (green) in co-culture with mCherry-CAAX MTLn3 tumor cells (red) showing heterotypic TNT formed by a macrophage towards a tumor cell. Duration of original sequence at least 30 mins. Magnification 60X, 2X2 binning. Frame interval: 10 s. Scale bar = 10 μm. Analysis performed with ImageJ (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997-2016).