Movie 5

Time-lapse imaging of GFP-CAAX RAW/LR5 macrophages (green) in co-culture with mCherry-CAAX MTLn3 tumor cells (red) showing heterotypic TNT formed by a macrophage towards a tumor cell. Duration of original sequence at least 30 mins. Magnification 60X, 2X2 binning. Frame interval: 10 s. Scale bar = 10 μm. Analysis performed with ImageJ (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997-2016).

Tunneling nanotubes, a novel mode of tumor cell–macrophage communication in tumor cell invasion

Samer J. Hanna, Kessler McCoy-Simandle, Edison Leung, Alessandro Genna, John Condeelis, and Dianne Cox

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.223321