To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 5 PI3K2(KE)-Halo-TMR & RBDRaf1-GFP/Ax2 (TIRF). Time-lapse TIRF images show PI3K2K857, 858E-Halo-TMR (left), RBDRaf1-GFP (center) and merged (right), corresponding to Fig. 3A. Excitable dynamics of Ras triggers spontaneous symmetry breaking of PIP3 signaling in motile cells Seiya Fukushima, Satomi Matsuoka, and Masahiro Ueda J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.224121 Download Original Video