Movie 7

PTEN-Halo-TMR & PHD_AKT/PKB-GFP /Ax2, PTEN-Halo-TMR & GFP-Nodulin /Ax2, RFP-Nodulin & RBD_Raf1-GFP /Ax2, PI3K2-Halo-TMR & GFP-Nodulin /Ax2, PTEN-Halo-TMR & RBD_Raf1-GFP /Ax2, PI3K2-Halo-TMR & PTEN-GFP /Ax2 (TIRF). Time-lapse TIRF images show RFP or Halo-TMR conjugated proteins (left), GFP conjugated proteins (center) and merged (right), corresponding to S5 and Fig. S4.