Movie 2

Loss of ena but not dia results in loss of nearly all lamellipodial bundles. Lamellipodial bundling in control, ena and dia M/Z macrophages expressing LifeAct-GFP (GREEN). A rare, probing ena actin bundle/filopod is shown. Images were acquired with spinning disc confocal microscopy (Perkin Elmer Ultraview) every 30 s. The scale bar is 10 μm. The movie frame rate is 4 frames/s.