Movie 4

Ena over-expression utilises fimbrin and/or forked to restore lamellipodial bundling in absence of fascin. Actin bundling (LifeAct-GFP, GREEN) in sn or fim, f, sn triple mutants ±Ena over-expression. Images were acquired with spinning disc confocal microscopy (Perkin Elmer Ultraview) every 30 s. The scale bar is 10 μm. The movie frame rate is 4 frames/s.