Movie 1

PkbR1-GFP is present on macropinosomes
PkbA-/PkbR1- cell overexpressing PkbR1-GFP (yellow) in TRITC-dextran containing media (magenta). PkbR1-GFP is present all over the cell membrane, including the macropinocytic cup, which is internalised and PkbR1-GFP remains on the endosome for a time after internalisation. Movie was taken at one frame per second on a Zeiss 710 microscope.