Movie 10

Polar body cytokinesis in sec-6 (RNAi) worms: Time lapse imaging showing first polar body extrusion in sec-6 (RNAi) C. elegans embryo imaged in utero. Polar body nucleus can be seen even after 8 minutes post-anaphase. Cellularization of polar body begins around this time with furrow ingression, but is not completed and finally relapses, leading to the internalization of the separated homologous chromosomes and increasing the ploidy of the embryo. Membrane is marked in green with PH::GFP and chromatin in red with mCherry::H2B.

The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge

Harsh Kumar, Kumari Pushpa, Amrita Kumari, Kuldeep Verma, Rajaiah Pergu, and Sivaram V. S. Mylavarapu

J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.226001