To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 3 The video shows a control GFP siRNA transfected U2OS cell achieving successful abscission. Frames were captured every 5 min. The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge Harsh Kumar, Kumari Pushpa, Amrita Kumari, Kuldeep Verma, Rajaiah Pergu, and Sivaram V. S. Mylavarapu J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.226001 Download Original Video