To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 2 Actin tail in A22 conditions.Time-lapse of HeLa cells transfected with LifeAct-mCherry and infected with S. flexneri GFP for 40 min and A22 was added for further 2 h. Infected cells were transferred to the microscope and imaged every 10 sec. Scale bar = 1 μm. Shigella MreB promotes polar IcsA positioning for actin tail formation Sina Krokowski, Sharanjeet Atwal, Damián Lobato-Márquez, Arnaud Chastanet, Rut Carballido-López, Jeanne Salje, and Serge Mostowy J Cell Sci 2019. 132:None-None; doi: 10.1242/jcs.226217 Download Original Video