Movie 2

Related to Figure 2A, E. Time-lapse video for Tln1-/-Tln2-/- cells expressing the reorganized talin proteins or the ΔR13-DD protein. Cells expressing different talin proteins were plated on a fibronectincoated polystyrene plate and allowed to attach to the surface for 120 minutes before imaging. Time-lapse series was collected at a 120 seconds interval for 12 hours. The size of each field is 200 μm × 200 μm. Note how the cells expressing the ΔR1-12 + C-terminal R1-3 protein have a non-polarized phenotype identical to cells expressing the ΔR1-12 form.