Movie 3

Related to Figure 2I. Time-lapse video for Tln1-/-Tln2-/- cells expressing the destabilized and tandem talin proteins presented. Cells expressing different talin proteins were plated on a fibronectin-coated polystyrene plate and allowed to attach to the surface for 120 minutes before imaging. Time-lapse series was collected at a 360 seconds interval for 12 hours. The size of each field is 200 μm × 200 μm. Note how the destabilization of the talin R3 subdomain by point mutations (ΔR4-12 (4S) talin mutant) inhibits cell spreading, polarization and migration. However, this effect is rescued by the addition of non-destabilized talin rod subdomains R1-3 into a tandem configuration (ΔR4-12 (4S) + R1-3 and ΔR4-12 (4S) + 2xR1-3 tandem proteins).