Nocodazole treatment disrupted EGFP-Rab10-tubules (related to Figure 3). HeLaM cells stably expressing EGFP-Rab10 were treated with 5 μg/ml nocodazole and imaged by confocal microscopy. Each time point was imaged as a z-stack (20 slices) of 0.5 μm intervals, and then maximum intensity projections of the z-stacks were acquired. The times indicated are relative to the time at the start of the video. Scale bar, 20 μm.