Movie 2

Breast carcinoma cell in G1, S/G2/M on fluorescent ECM.
MDA-MB-231-FUCCI-mCerulean3-Lifeact7 cell in G1 (red nucleus) assembles invadopodia (green puncta) which degrade the underlying fluorescent gelatin (gray, right panel). Cell in S/G2 assembles invadopodia precursors or degrade ECM. Images were acquired every 10 minutes for 10 hours. Scale bar 20 μm.