Movie 5

Breast carcinoma cell transitioning through G1, S and G2 on fluorescent ECM.
MDA-MB-231-FUCCI-mCerulean3-Lifeact7 cell in G1 (red nucleus) forms invadopodia (cyan puncta) and degrades the underlying fluorescent gelatin (green for gelatin and black for degraded gelatin puncta, middle panel) only during G1. Right panel shows live plot of accumulating gelatin degradation. Images were acquired every hour for 50 hours. Scale bar 10 μm.